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Our goal: To help you select your best clone, mutant or growth medium:
- our sandwich covers convert wells of 24 and 96 well microplates into high-quality mini-bioreactors with equal properties, and so put an end to well-to-well variations due to unequal (and too high) evaporation rates
- our clamp systems press the sandwich covers tightly upon the microplates, and so allow orbital shaking as vigorous as you wish
- our databases (videos of hydrodynamics, and tables of oxygen transfer rates) allow you to easily select the right shaking conditions for your project
- our 'how we work' section provides a step plan how we can assist you efficiently
System Duetz
- A mature, easy-to-use, and cost-effective technology platform for the rapid handling and cultivation in microplates of large numbers of microbial strains, clonal libraries, mutant banks, CHO-cells, etc.
- The system enables the growth in microplates of thousands of strains simultaneously with a minimum of repetitive handlings: all handling is performed in parallel for sets of 24 or 96 strains.
- Same quality of growth as in Erlenmeyer flasks in terms of oxygen transfer rates and reproducibility. See our pages on the hydrodynamics inside the wells during orbital shaking (see also our streaming video website: www.videosduetz.com.
- 24 square deepwell plates (2.5 - 4 ml cultures)
- 96 square deepwell and half deepwell plates (0.3 - 0.75 ml cultures)
- 24 round lowwell plates (0.75-1.5 ml cultures)
- 96 round lowwell plates (0.1 - 0.2 ml cultures)
- The system was originally developped and validated at the Institute of Biotechnology of the ETH Zurich: read the key publication from the year 2000 in Applied and Environmental Microbiology (Duetz et al. 2000, pdf-file [146 kb]).
Application areas:
- Cultivation of CHO cells and other animal cell lines
- Discovery: high-throughput screening for new enzyme activities or formation of secondary metabolites (heterogeneous culture collections)
- Screening and distribution of clone libraries in E. coli or yeasts
- High-throughput screening for high-activity or high-productivity mutants
- Comparative studies e.g. clinical isolates
- Growth medium optimization studies
For specific application examples of current users, click here
The system has been extensively tested and validated for a range of bacterial strains and streptomycetes (see literature for details). For use for yeasts and fungi, please contact us.
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News / new products:
- March 2012. New comprehensive lists of 24 and 96 well microplates most suitable for cultivations.
- February 2012: new polystyrene 96 square half-deepwell MTP available to customers (designed for primary screenings of both microbial mutant libraries and clone selection). Well volume: 1.1 ml, pyramid bottom. Recommended culture volume: 0.25-0.4 ml.
Our universal clamp system CR1800 with:
left: a stack of 3 new 96-well MTPs + covers CR1396
right: one 24 deepwell MTP + cover CR1224a
Our deepwell clamp system CR1700
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Main components:
- Sandwich covers convert all wells of microtiter plates into individual "mini-reactors" suitable for high-frequency orbital shaking, at high oxygen transfer rates, and without well-to-well variations.
- Cover clamps ensure that the deepwell plate and its sandwich cover are clamped together tightly, thereby preventing well-to-well contamination
- The cryo-replicator*: a precision spring-loaded device for the simultaneous and reproducible sampling of 96 frozen glycerol stocks. With the CRYO-REPLICATOR culture stocks remain viable for years because they can remain frozen.
- The cryo-replicator press is a guiding system ensuring accuracy during the replication of sets of strains
* EP1192438
General procedure used to generate living suspended cultures, starting from a master-plate with 96 individual strains stored at - 80 ºC in the presence of 15% (v/v) glycerol
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Our cryo-replicator press CR1100
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