Microplates compatible with the Growth profiler:
CR1424f:
Polystyrene 24 roundwell microplates from Porvair
(used for shear-sensitive strains and cells)
•
1 ml culture volume per well
•
round wells, so lower shear forces
•
at 250 rpm, OTR ~30 mmol O2/min
•
accurate biomass determination from
OD 0.05 up till OD 15
•
to be used with sandwich cover CR1324e
CR1424dg:
Polystyrene greyish-white 24 square well microplate
•
2.5 or 3 ml culture volume per well
•
at 250 rpm, OTR ~40 mmol O2/min
•
accurate biomass determination from OD0.05 up till OD32
•
response curve saturates above OD30
•
to be used with sandwich cover CR1224f or CR1224g
CR1496dg:
Polystyrene greyish-white square 96-half-deepwell microplate
•
250 μl culture volume per well
•
at 250 rpm, OTR = ~40 mmol O2/min
•
extra turbulenence due to square shape prevents settling of
cells, see video
•
accurate biomass determination from OD 0.05 up till OD 10
•
OD 10-32 measurable (though less accurate)
•
light greyish color of walls (a tiny concentration of carbon black
is added to the white plastic)
fully eliminates cross-talk
•
no leachables (transparent bottom is laser-welded, not glued)
•
to be used with sandwich cover CR1396b
•
high OTR allows exponential growth of baker's yeast up to OD
6-7 (see growth curve below)
•
applicable for both bacterial and yeast cultures
•
replaces the (discontinued) white-walled CR1496d MTPs, that
had some cross-talk under certain conditions,
and had a poorer response at high OD
•
has a similar response curve as CR1496d, but not identical, so
for the most accurate results,
a new strain calibration is required (see graph right-below)
Wells of CR1496dg filled with various densities of Baker's yeast: the
calibration/response curve below is based on these images
Growth curve of Baker's yeast on (filter-sterilised)
YPD medium (250 ul volume, 250 rpm)
Respose curve of Baker's yeast in CR1496dg MTPs based om images above; such curves
are used to convert the measured “green-values” into OD- equivalents in the
Growthviewer software
Comparison of respose curves for Baker's yeast for CR1496dg and CR1496d (the latter
being discontinued)
Comparison of respose curves for Baker's yeast for CR1424e and CR1496f
CR1496dc:
•
as CR1496dg (see above), but made from Topas COC 8007
•
has a better resistance to some solvents
•
contains a higher percentage of carbon black, resulting in a better
response at higher OD values
MTPs with an increased tolerance to organic solvents:
CR1496cp:
•
half-deepwell MTP made from polypropylene, containing
only low concentrations of additives
•
has a very thin round bottom (90% transparent)
•
resistant to practically all organic solvents
•
response curve comparable to that of CR1496dg
CR1424g:
•
24 glass tubes in a 3D printed nylon rack
