An efficient way to assist you in making the right choices:
1) You send us a description of your project/application by E-mail ( info(at)enzyscreen.com ), preferably with the following info:
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What type of strain / cell line is being used
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The type of product of interest (e.g. an enzyme, an anitibody, a secondary metabolite, etc)
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The cultivation time (number of days)
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The growth temperature
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Your inoculation procedure
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What the minimally required culture volume is
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What minimal accuracy is required (maximal variability of independent duplicates)
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The number of clones/mutants/conditions to be screened
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The type of orbital shakers available in your lab (what trade mark, what shaking diameter)
2) We respond by E-mail or by phoning. Together, we establish what combinations of culture volume - microplate - sandwich cover - clamp
system - shaking conditions is the most suitable for your purpose.
3) We supply you with a such a system which you test on a limited number of strains/mutants/cell lines, mainly to assess the reproducibility
(standard deviation among independent duplicates), the productivity in comparison to your present cultivation systems (e.g. shake flasks or
tubes).
4) In a phonecall, we together evaluate these initial tests, and decide which system to use for the full screenings
Note: It is our experience (having worked in this way with already more than 100 customers) that it is worth to spend enough time on the
optimization of the inoculation and screening procedure: it is often better to spend 8 weeks on optimization and 2 weeks on the screening
itself than the other way around..