Growth Profiler 960
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Generates growth curves of up to 960 microbial cultures (10 MTPs) in
microtiter plates
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Because oxygen-transfer rates of 40 mmol/L. h are readily reached (at 225
rpm/50mm), exponential growth (no O2-limitation) occurs up till OD600
values of 3 -10 (depending on the strain and its specific oxygen demand),
which allows an accurate determination of maximal growth rates (see
example curves at the bottom of this page)
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Shaker unit can be set to slow down (e.g. every 30 minutes) to 30 rpm for
a few seconds: in these few seconds the culture comes temporarily to a
rest (surface becomes close to horizontal), and the 10 cameras make
photos of the bottom of the (transparent) wells.
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Image analysis software (included) quantifies the cell density, and
produces growth curves for all 960 strains (see example growth curves at
the bottom of this page; in this case, the biomass levels are expressed in
OD600 equivalents)
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Biomass concentrations can be quantified up to 20 g dry wt / L
(corresponding to approx OD600 = 60) using a previously prepared
calibration curve) when grey or black-walled MTPs are used
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Biomass levels down to OD600 values of 0.05 or 0.1 (below 0.05 g dry
wt/L) can be measured (see example growth curves at the bottom of this
page) when white-walled MTPs are used.
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Compatible with our standard microtiter plates and sandwich covers (that
prevent cross-contamination and excessive evaporation)
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Clamp system identical to the system used for our universal clamps
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Incubator with heater allows temperature control between 20 and 40
degrees Celsius.
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Suitable for bacterial and cell cultures
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Until now only supplied to selected customers/collaborators, but available
from stock to everyone soon (May 2016)
Growth profiler 960
Example photo of a collection of wild type strains growth
Below left:
Example growth curves of a bacterial strain growing at a culture
volume of 300 ul in a 96 half square deepwell plate CR1496d,
shaken at 50 mm/225 rpm
Below right:
Enlargement of the growth curve on the left, in the first three
hours of cultivation, showing that changes in OD600 values of
0.05 can be easily detected
For the automated quantification of growth rates, we recommend
to use the “Growth rate 5.0” software from Bellingham Research