For general info, technical questions, product and
price lists, and quotations,
please contact us by E-mail:
•
info(at)enzyscreen(dot)com
For information on the Growth profiler, and if you
wish to discuss a specific application, please contact
Wouter Duetz by E-mail:
•
duetz(at)enzyscreen(dot)com
Enzyscreen BV
VAT number: NL8104.60.348.B01
Chambre of Commerce : 34164153
Kuhner AG, Basel Switserland
(specialised in high-quality orbital shakers)
Collaboration partners
We continuously develop new products, and among our collaboration partners are:
EnzyScreen BV
Herenweg 29R
2105 MB Heemstede
The Netherlands
Tel: +31 23 8222655
Contact/About us
Bellingham Research Institute is specialized in software for the quantification
of growth rates
About us
Enzyscreen BV has its production and R&D facilities in Heemstede, The
Netherlands, close to Amsterdam international airport. From here, we ship
all our products (exclusively by FEDEX) to our 200+ worldwide customers at
universities, and in the biotech and pharma industry
Enzyscreen was founded in 2002 as a spin-off company of the Institute of
Biotechnology of the ETH Zurich , and intitially mainly performed contract-
screenings for new enzymes (in a collection of 1850 wild-type strains).
Since 2008, Enzyscreen fully focusses on further developping, selling, and
implementing their technology platform for the miniaturised screening of
large microbial collection, in microtiter plate format.
Wouter Duetz (CEO and cofounder) studied Physics, Chemistry, Biochemistry and
Microbiology at the University of Amsterdam, before working for 10 years on
oxygenases involved in the microbiological degradation of aromatic pollutants. In the
subsequent 5 years, he lectured enzymology and biotechnology at the ETH Zurich
and headed a research group focussed on high-throughput screening for new
biocatalysts, in close collaboration with Prof. Bernard Witholt.
Specific expertises:
- miniaturised cultivation systems for bacteria, yeasts, and aninal cells
(such as CHO-cells)
- mixing and oxygen transfer rates in various types of microplates, under various
shaking conditions
- gas-liquid mass transfer
- measurement and precise quantification of maximal growth rates
- anearobic cultivations of bacteria and yeasts
- regio- and enantiospecific hydroxylations
- physics and engineering of orbital shaking systems
Publications (selection of totally 42, in descending order of
citation numbers), h-index 24:
Duetz, W.A., Rüedi, L., Hermann, R. O'Connor, K, Büchs, J., and Witholt, B.
(2000). Methods for intense aeration, growth, storage, and replication of
bacterial strains in microtiter plates. Applied and Environmental Microbiology
66:2641-2646 (230 citations)
Duetz, W.A., van Beilen, J.B. and Witholt, B (2001). Using proteins in their
natural environment: potential and limitations of microbial whole-cell
hydroxylations in applied biocatalysis. Curr. Opin. Biotechnol., 12:419–425
(197 citations)
Duetz, W.A., Bouwmeester, H., van Beilen, J.B. and Witholt, B. (March 2003)
Biotransformation of limonene by bacteria, fungi, yeasts, and plants. Appl.
Microbiol. Biotechnol. 61:269–277 (163 citations)
Duetz,
W.A.
(2007)
Microtiter
plates
as
mini-bioreactors:
miniaturization
of
fermentation
methods,
TRENDS
in
Microbiology
15
(10):
470-475
(147
citations)
Duetz,
W.A.
and
Witholt,
B.
(2001).
Effectiveness
of
orbital
shaking
for
the
aeration
of
suspended
bacterial
cultures
in
square
deepwell
microtiter
plates.
Biochemical Engineering Journal, 7: 113-115 (77 citations)
Duetz,
WA,
Witholt,
B.
(2004)
Oxygen
transfer
by
orbital
shaking
of
square
vessels
and
deepwell
microtiter
plates
of
various
dimensions.
Biochemical
Engineering Journal 17: 181–185 (71 citations)
Duetz,
W.A.,
Fjällman,
A.H.M.,
Ren,
S.,
Jourdat,
C.,
and
Witholt,
B.
(2001)
Biotransformation
of
D-limonene
to
(+)
trans
-carveol
by
toluene-grown
cells
of
Rhodococcus
opacus
PWD4.
Appl.
Environ.
Microbiol.,
67:
2829-2832
(56
citations)
Minas,
W.,
Bailey,
J.E.,
Duetz,
W.A.
(2001)
Streptomycetes
in
microcultures:
growth,
production
of
secondary
metabolites,
and
storage
and
retrieval
in
the
96-well
format.
Antonie
van
Leeuwenhoek,
78
(3-4):
297-305
(51
citations)
Duetz,
W.A.,
Chase,
M.,
Bills,
G.
(2010)
Miniaturization
of
Fermentations.
Chapter
8,
ASM
Manual
of
Industrial
Microbiology
and
Biotechnology,
R.
H.
Baltz, J. E. Davies, A. L. Demain (eds)
Patents:
Duetz,
W.A.,
and
Witholt,
B
(2000)
Method
and
apparatus
for
sampling
frozen
cultures
of
microorganisms
and/or
eukariotic
cells
simultaneously
and
independently of each other, Patent Application PCT/NL00/00426 .
Duetz,
W.A.,
Jourdat,
C.,
Witholt,
B.
(2000)
Process
for
the
preparation
of
trans-carveol and/or carvone, PCT application November 2000
Duetz,
W.A.,
Jourdat,
C.,
Witholt,
B.
(2001)
Process
for
the
preparation
of
perillyl alcohol, PCT application. February 2001
Duetz,
W.A.
(November
2009)
An
apparatus
and
a
method
for
investigation
of
microtiter
plates
subjected
to
orbital
shaking.
PCT
application
PCT/EP2009/062858 (May 2011). EP application # 09783713.2
Selection of other publications (chronological):
Van
der
Bend,
R.L.,
Duetz
W.,
Colen,
A.M.,
Van
Dam,K.,
Berden
J.A.
(1985)
Differential
effects
of
triphenyltin
and
8-azido-ATP
on
the
ATP
synthesis,
ATP-Pi
exchange,
and
ATP
hydrolysis
in
liposomes
containing
ATP
synthase
and
bacteriorhodopsin. Arch. Biochem. Biophys. 241: 461-71
Breure,
A.M.,
Duetz,
W.A.,
Zoutberg,
G.R.,
Mulder,
R.,
Andel,
J.G.
van
(1986)
Measurement
of
the
rate
of
hydrogen
production
by
Clostridium
butyricum
using
an
amperometric method. FEMS Microbiol. Lett. 33, 289-292
Duetz,
W.A.,
Andel,
J.G.
van
(1991)
Stability
of
TOL
plasmid
pWW0
in
Pseudomonas
putida
mt-2
under
non-selective
conditions
in
continuous
culture.
J.
Gen.
Microbiol
137, 1369-1374
Duetz,
W.A.,
Winson,
M.K.,
Andel,
J.G.
van,
Williams,
P.A.
(1991)
Mathematical
analysis
of
catabolic
function
loss
in
a
population
of
Pseudomonas
putida
mt-2
during non-limited growth on benzoate. J. Gen. Microbiol. 137, 1363-1368
Duetz,
W.A.,
Marqués,
S.,
Jong,
C.
de,
Ramos,
J.L.,
Andel,
J.G.
van
(1994)
Inducibility
of
the
TOL
Catabolic
pathway
in
Pseudomonas
putida
(pWW0)
growing
on
succinate
in
continuous
culture:
Evidence
of
carbon
catabolite
repression
control.
J. Bacteriol. 176:2354-2361
Duetz,
W.A.,
De
Jong,
C.,
Williams,
P.A.
and
Van
Andel,
J.G.
(1994)
Competition
in
chemostat
between
Pseudomonas
strains
using
different
pathways
for
the
degradation of toluene. Applied and Environmental Microbiology 60:2858-2863.
Duetz,
W.A.,
Marques,
S.,
Wind,
B.,
Ramos,
J.L.
Van
Andel,
J.G.
(1996)
Catabolite
repression
of
the
toluene
degradation
pathway
in
Pseudomonas
putida
(pWW0)
under
various
limitations
in
chemostat
culture.
Applied
and
Environmental
Microbiology 62:601-606
Duetz,
W.A.,
Wind,
B.,
Kamp,
M.,
Van
Andel,
J.G.
(1997)
Effect
of
growth
rate,
nutrient
limitation
and
succinate
on
expression
of
TOL
pathway
enzymes
in
response
to
m
-xylene
in
chemostat
cultures
of
Pseudomonas
putida
(pWW0).
Microbiology
143: 2331-2338
Filonov,
A.E.,
Duetz,
W.A.,
Karpov,
A.V.,
Gaiazov-R.R.,
Kosheleva,
I.A.,
Breure,
A.M.,
Filonova,
I.F.,
Van
Andel,
J.G.,
Boronin,
A.M.
(1997)
Competition
of
plasmid-bearing
Pseudomonas
putida
strains
catabolizing
naphthalene
via
various
pathways
in
chemostat culture. Applied Microbiology and Biotechnology 48: 493-498
Duetz,
W.A.,
Wind,
B.,
Van
Andel,
J.G.,
Barnes,
M.R.,
Williams,
P.A.,
Rutgers,
M.
(1998)
Biodegradation
kinetics
of
toluene,
m
-xylene,
p
-xylene
and
their
intermediates
through
the
upper
TOL
pathway
in
Pseudomonas
putida
(pWW0).
Microbiology, 144; 1669-1675
Duetz,
W.A.,
Wind,
B.,
Kamp,
M.,
Van
Andel,
J.G.
(1997)
Effect
of
growth
rate,
nutrient
limitation
and
succinate
on
expression
of
TOL
pathway
enzymes
in
response
to
m
-xylene
in
chemostat
cultures
of
Pseudomonas
putida
(pWW0).
Microbiology
143: 2331-2338
Duetz,
W.A.,
Wind,
B.,
Van
Andel,
J.G.,
Barnes,
M.R.,
Williams,
P.A.,
Rutgers,
M.
(1998)
Biodegradation
kinetics
of
toluene,
m
-xylene,
p
-xylene
and
their
intermediates
through
the
upper
TOL
pathway
in
Pseudomonas
putida
(pWW0).
Microbiology, 144; 1669-1675
Rashidi
H,
Akhtar
MT,
van
der
Kooy
F,
Verpoorte,
R,
and
Duetz,
W.A.
(2009)
Hydroxylation
and
further
oxidation
of
delta9-tetrahydrocannabinol
by
alkane-
degrading bacteria. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75:7135-7141
Publications
Contact
If you rather see this intoduction video dubbed in English please click here (Vimeo video)
Enzyscreen broadcasted on Dutch tv channel RTL-Z on the 16th of june 2019:
