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Choice of growth vessels for microorganisms and cell cultures. The information below may be helpful to decide which growth vessel is most appropriate for a specific purpose. Also included in this set of web-pages is information on the clamps and holders supplied from Kuhner AG.
Regular Erlenmeyer flasks
For all routine research work on house strains.
- moderate to high aeration rates (up to 40 mmol O2/L/h)
- low shear forces, low mixing intensity in bulk liquid, no splashing
- well reproducible growth curves
- same shaking patterns in flasks in range 25-5000 ml; easy scaling-up to culture volumes of 500 ml
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Microtiter plates
For the parallel cultivation of large numbers of strains, clones, and mutants.
Using our sandwich lids + clamps, square deepwell microtiter plates are a mature alternative for Erlenmeyer flasks when low culture volumes suffice.
- moderate to high aeration rates (up to 50 mmol O2/L/h)
- high mixing intensity; higher than in Erlenmeyer flasks, and therefore better mimicking of conditions in stirred-tank bioreactors (relevant in medium optimization, and mutant screening)
- no splashing
- low evaporation (10 ul per well per day at 50% relative humidity)
- well reproducible growth curves
- impressive time-savings if used in combination with our replication system
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Round bottles
For the cultivation of strains in the absence of exclange of headspace air
- The headspace volume of bottles is 2-3 fold higher than in Erlenmeyer flasks. Therefore, 2-3 fold more biomass can be generated using solely the oxygen present in the headspace (closed lid).
- suitable for strains that need a specific gas-atmosphere
- suitable for strains to be grown on volatile C-sources (e.g. alkanes, toluene)
- larger surface area during shaking and therefore higher oxygen-transfer rates than in Erlenmeyer flasks of similar size
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Square bottles
For e.g. the growth of strains on poorly soluble solids and organic liquids
- moderate to high aeration rates (up to 60 mmol O2/L/h)
- high mixing intensity; higher than in Erlenmeyer flasks, and therefore better mimicking of conditions in stirred-tank bioreactors (relevant in medium optimization, and mutant screening)
- high turbulence and splashing at high shaking frequncies, especially with bottle volumes over 100 ml
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Tubes
For the cultivation of eukaryotic cell lines
- low aeration rates
- low shear forces
- low mixing intensity
- easy to handle in parallel
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