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News / new products:
- End January 2012: new polystyrene 96 square half-deepwell MTP available to customers (developped together with 4titude, designed for primary screenings of both microbial mutant libraries and clone selection). Well volume: 1.1 ml, pyramid bottom. Recommended culture volume: 0.25-0.4 ml. Three MTPs + covers can be stacked on top of each other in our regular lowwell clamps. Compatible with our universal covers CR1396. Will be produced from ultraclean polystyrene, and will be supplied individually wrapped, gamma-irradiated, ready for use. For more info, contact us.
- 11-13 October 2011, Enzyscreen at Biotechnica in Hannover (Hall 9, booth G4, right at the main entrance of Hall 9): all our regular products as well as our new Growth profiler 1152 will be on show.
- 1 August 2011: Enzyscreen moves into a new - larger - building in Haarlem (Waardepolder, close to city center), and now fully focusses on the development and production of its screening and cultivation equipment.
- April 2011: new introductory video, available via Youtube (also in HD-version), and also via our own streaming video website: www.videoduetz.com (in quicktime format).
- February 2011: The first large batch of polystyrene 24 square-deepwell microtiter plates (a co-product of Applikon and Enzyscreen) available to our customers. These new MTPs have a pyramid bottom and a volume of 11 ml per well, are made of - ultrapure - polystyrene, and are supplied in boxes of 20, individually wrapped, and gamma-irradiated. In essence they are a disposable version (same well shape and volume) of the polypropylene (re-usable) MTPs from HJ-Bioanalytik that we supply as well. In March, the first batch op similar polystyrene MTPs with a transparant bottom will be commercially available (for use with Presens readers, and with our Growth profiler 1152).
Polystyrene 24 square deepwell MTPs, transparent and white version
System Duetz
- An integrated, easy-to-use, and cost-effective technology platform for the rapid handling and cultivation in microplates of large numbers of microbial strains, clonal libraries, mutant banks, and cell lines.
- The system enables the growth in microplates of thousands of strains simultaneously with a minimum of repetitive handlings: all handling is performed in parallel for sets of 24 or 96 strains.
- Users report time-savings in strain handling of typically a factor 10.
- Same quality of growth as in Erlenmeyer flasks in terms of oxygen transfer rates and reproducibility: See it for yourself; view the technical data, photos and movies showing the hydrodynamics inside the wells during orbital shaking (see also our streaming video website: www.videosduetz.com.
- Sandwich covers are now available for:
- 24 square deepwell plates (2.5 - 4 ml cultures)
- 96 square deepwell plates (0.5 - 1 ml cultures)
- 24 round lowwell plates (0.75-1.5 ml cultures)
- 96 round lowwell plates (0.1 - 0.2 ml cultures)
- The system was originally developped and validated at the Institute of Biotechnology of the ETH Zurich: read the key publication in Applied and Environmental Microbiology (Duetz et al. 2000, pdf-file [146 kb]).
Application areas:
- Cultivation of CHO cells and other animal cell lines
- Discovery: high-throughput screening for new enzyme activities or formation of secondary metabolites (heterogeneous culture collections)
- Screening and distribution of clone libraries in E. coli or yeasts
- High-throughput screening for high-activity or high-productivity mutants
- Comparative studies e.g. clinical isolates
- Growth medium optimization studies
For specific application examples of current users, click here
The system has been extensively tested and validated for a range of bacterial strains and streptomycetes (see literature for details). For use for yeasts and fungi, please contact us.
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new polystyrene 96 square half-deepwell MTP,
available to customers in December
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Main components:
- Sandwich covers convert all wells of microtiter plates into individual "mini-reactors" suitable for high-frequency orbital shaking, at high oxygen transfer rates, and without well-to-well variations.
- Cover clamps ensure that the deepwell plate and its sandwich cover are clamped together tightly, thereby preventing well-to-well contamination
- The cryo-replicator*: a precision spring-loaded device for the simultaneous and reproducible sampling of 96 frozen glycerol stocks. With the CRYO-REPLICATOR culture stocks remain viable for years because they can remain frozen.
- The cryo-replicator press is a guiding system ensuring accuracy during the replication of sets of strains
* EP1192438
General procedure used to generate living suspended cultures, starting from a master-plate with 96 individual strains stored at - 80 ºC in the presence of 15% (v/v) glycerol
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