Gastight box (with clamp inside)
The gastight box with a clamp inside (CR1601) enables microtiter plate fermentations requiring a specific gas-atmosphere (e.g. a higher CO
2 concentration, or anearobic cultivations). The clamp system inside is identical to the clamp system for lowwell MTPs (see
Lowwell plate covers and clamps), but this system can also harbour deepwell plates with sandwich covers.
The gastight box can be mounted onto shaking platforms of various trademarks, and has been constructed to withstand continuous shaking up to 300 rpm at an amplitude of 25 mm, and 225 rpm at an amplitude of 50 mm. Because of these limitations in orbital shaking conditions, we advise to work either with 24 well MTPs and covers or with square lowwell MTPs (from Matrical) in combination with our universal covers for 96-well MTPs (
CR 1597)
The airtight box has a gas inlet and a gas outlet (both via a ball-valve), that is compatible with standard 6 mm push-in connectors.
The box is gastight (using an EPDM rubber sealing between the perspex box and the carbon groundplate), but is not resistant to overpressure or underpressure more than 0.1 bar. Therefore, the box can not be made anaerobic using vacuum cycles, but has to be flushed instead. The dimensions (groundplate 220x194 mm, total height including ball valves 220mm) allow the transfer in and out most anearobic chambers (via an airlock: vacuum cycles can be used provided the valves of the box are open, or the perspex box and the alu groundplate are transferred into the anaerobic chamber separately).
Continuous flushing during shaking is feasible using PTFE tubing (outside diameter 6 mm).
The total weight (including microtiter plates and covers) is approximately 2.5 kg, which implies that - also due to the relatively high heavy point - maximally 4-6 boxes can be fitted onto one shaking platform (depending on the shaker model).
The gastight box can harbour either two deepwell MTPs with sandwich covers, or six lowwell MTPs with sandwich covers.
Application areas:
- anaerobic cultivations (e.g. in biofuel production)
- high-CO2 cultivations (e.g. mammalian cells)
- long-term cultivations at 100% humidity (to prevent culture volume loss due to evaporation alltogether)
