Hydrodynamics

 

Photos of isolated wells from various types of microtiter plates during orbital shaking at 300 rpm, showing the large effect of the shaking diameter ("throw") on the degree of mixing, especially for the smaller cultures.

 

 

 

 

 

 

Videos (50 times slowed down) of cultures in isolated wells of various types of microtiter plates:

 

 

 

96-lowwell, 300 rpm, 50 mmm shaking amplitude, cultures

of Pseudomonas putida in Nutrient Broth (click here to view

or download corresponding video)

 

 

 

 

 

 

24-lowwell (diameter 16 mm), 300 rpm, 50 mmm shaking amplitude, 0.5 and 0.75 ml aqueous medium with colorant (click here to view or download corresponding video)

 

 

 

96-square deepwell (8x8 mm), 300 rpm, 50 mm shaking amplitude, 0.75 ml aqueous medium with colorant (click here to view or download corresponding video)

 

 

 

 

96-and 24-square deepwells (8x8 mm and 17x17 mm resepctively), 300 rpm, 50 mm shaking amplitude, 0.75 ml and 3 ml aqueous medium with colorant (click here to view or download corresponding video)

 

 

 

96-and 24-square deepwells (8x8 mm and 17x17 mm resepctively), 300 rpm, 25 mm shaking amplitude, 0.75 ml and 3 ml aqueous medium with colorant (click here to view or download corresponding video)