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Upcoming talks on cultivation in microplates:
- 14 to 16 March 2012, Berlin, Three-day symposium about development and application of bioprocesses in industrial scale, talk Wouter Duetz, Tibor Anderlei & Markus Kuhner (Kuhner AG, Basel, Switzerland) entitled: "Shaken bioreactors with culture volumes of 0.1 ml to 200 liter: effects of scale, vessel geometry and shaking parameters on kLa values, mixing times and hydrodynamics" (www.ifgb.de/bioproscale)
- August 12-16, 2012, Washington DC, SIM annual meeting, workshop scale up - scale down & screening. Talk by Wouter Duetz, details follow later (www.simhq.org/)
Recent talks on cultivation in microtiter plates:
- 29 September 2011, ECAB 2011, Berlin, Shaking Technology-Session, talk Wouter Duetz entitled: "How to turn wells of microtiter plates into high-quality "mini-bioreactors", see: www.ecce2011.de/ecab2011.html
- 1-5 August, 2010, SIM, Annual Meeting and Exhibition, talk Wouter Duetz entitled: "Cultivation in various types of microtiter plates, and quantification of cell mass using flatbed scanning and image analysis software"
- SGM meeting Edinburgh, 7-11 September 2009, "Cultivation and biotransformation in microtiter plates", by Wouter Duetz (part of a session organized by Krist Gerneay, called "Cultivating and sensing microbes in micro-scale devices" , for a complete programme of this session, see: www.sgm.ac.uk/meetings/MTGPAGES/hw0908.cfm
- RAFT meeting, San Diego, 8-11 November 2009 "Cultivation in microtiter plates, quantification of biomass using flatbed scanning technology" by Wouter Duetz". This talk is part of a session called "New fermentation Technology" organized by Jeff Keating and Wouter Duetz. For a complete programme, see www.simhq.org/meetings/meetings.aspx
Relevant literature:
Recent review articles & patent applications:
- Duetz, W.A., Chase, M., Bills, G. (2010) Miniaturization of Fermentations. Chapter 8, ASM Manual of Industrial Microbiology and Biotechnology, R. H. Baltz, J. E. Davies, A. L. Demain (eds)
- Duetz, W.A. November 2008. An apparatus and a method for investigation of microtiter plates subjected to orbital shaking. Patent 86185NL00 (note: this patent application concerns the application of flatbed scanning technology for the quantification of biomass in microtiter plate wells during a short interruption in their orbital shaking)
- Duetz, W.A. (2007) Microtiter plates as mini-bioreactors: miniaturization of fermentation methods, TRENDS in Microbiology 15 (10): 470-475
- Duetz, W.A., Kuhner M., Lohser R. (2006) Microbial and cell growth in microtiter plates, GENETIC ENGINEERING NEWS 26 (11): 44-46 JUN 1 2006
Key research article:
- Duetz, W.A., Rüedi, L., Hermann, R. O'Connor, K, Büchs, J., and Witholt, B. (2000). Methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates. Applied and Environmental Microbiology 66:2641-2646 (pdf-file, 146kb)
Other articles:
- Minas, W., Bailey, J.E., Duetz, W.A. (2000). Streptomycetes in microcultures: growth, production of secondary metabolites, and storage and retrieval in the 96-well format. Antonie van Leeuwenhoek, 78 (3-4): 297-305 (pdf-file, 213 kb)
- Clerval, R., Kühner, M., Li, Z., Minas, W., Fjällman, A., Witholt, B. and Duetz, W.A. (2000). The come-back of high-throughput screening of wild-type microbial strains through the use of miniaturised growth systems and LC-MS. Bioworld 6: 24-26
- Duetz, W.A. and Witholt, B. (2001). Effectiveness of orbital shaking for the aeration of suspended bacterial cultures in square deepwell microtiter plates. Biochemical Engineering Journal, 7: 113-115 (pdf-file, 94 kb)
- Duetz, W.A., Minas, W., Kuhner, M., Clerval, R., Fjallman, A.H.M., Witholt, B. (2001). Miniaturized microbial growth systems in screening. Bioworld 2: 8-10 (pdf-file, 2252 kb)
- Duetz, WA, Witholt, B. (2004). Oxygen transfer by orbital shaking of square vessels and deepwell microtiter plates of various dimensions. Biochemical Engineering Journal 17: 181-185 (pdf-file, 75 kb)
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