Relevant publications 2000 - 2004
Duetz, W.A., Rüedi, L., Hermann, R. O'Connor, K, Büchs, J., and Witholt, B. (2000). Methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates. Applied and Environmental Microbiology 66:2641-2646 (
pdf-file, 146kb)
Clerval, R., Kühner, M., Li, Z., Minas, W., Fjällman, A., Witholt, B. and Duetz, W.A. (2000). The come-back of high-throughput screening of wild-type microbial strains through the use of miniaturised growth systems and LC-MS. Bioworld 6: 24-26
Duetz, W.A. and Witholt, B. (2001). Effectiveness of orbital shaking for the aeration of suspended bacterial cultures in square deepwell microtiter plates. Biochemical Engineering Journal, 7: 113-115 (
pdf-file, 94 kb)
Minas, W., Bailey, J.E., Duetz, W.A. (2000). Streptomycetes in microcultures: growth, production of secondary metabolites, and storage and retrieval in the 96-well format. Antonie van Leeuwenhoek, 78 (3-4): 297-305 (
pdf-file, 213 kb)
O'Connor, K.E., Witholt, B., Duetz, W.A. (2001).
p-Hydroxyphenylacetic acid metabolism in Pseudomonas putida F6. J. Bacteriol. 183: 928-933 (
pdf-file, 96 kb)
Duetz, W.A., Minas, W., Kuhner, M., Clerval, R., Fjallman, A.H.M., Witholt, B. (2001). Miniaturized microbial growth systems in screening. Bioworld 2: 8-10 (
pdf-file, 2252 kb)
Duetz, W.A., Fjällman, A.H.M., Ren, S., Jourdat, C., and Witholt, B. (2001). Biotransformation of D-limonene to (+) trans-carveol by toluene-grown cells of
Rhodococcus opacus PWD4. Appl. Environ. Microbiol., 67: 2829-2832 (
pdf-file, 80 kb)
Duetz, W.A., van Beilen, J.B. and Witholt, B (2001). Using proteins in their natural environment: potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis. Curr. Opin. Biotechnol., 12:419–425 (
pdf-file, 80 kb)
Li Z, van Beilen JB, Duetz WA, Schmid A, de Raadt A, Griengl H, Witholt B. (2002) Oxidative biotransformations using oxygenases. Curr. Opin. Chem. Biol. 6 (2): 136-144
Duetz, W.A., Bouwmeester, H. van Beilen, J.B. and Witholt, B. (March 2003) Biotransformation of limonene by bacteria, fungi, yeasts, and plants. Appl. Microbiol. Biotechnol. 61:269–277 (
pdf-file, 297 kb)
Spain, J.C., Hishino, S.F., Witholt, B., Tan, L.S., Duetz, W.A. (2003) Production of 6-Phenylacetylene Picolinic Acid from Diphenylacetylene by a Toluene-Degrading
Acinetobacter Strain. Appl. Environ. Microbiol. 69:4037-4042 (
pdf-file, 567 kb)
Duetz, WA, Witholt, B. Oxygen transfer by orbital shaking of square vessels and deepwell microtiter plates of various dimensions (2004). Biochemical Engineering Journal 17: 181-185 (
pdf-file, 76 kb)
Zhang, J, Duetz, W.A., Witholt, B., and Li, Z. (2004). Rapid identification of new bacterial alcohol dehydrogenases for (R)- and (S)-enantioselective reduction of ß-ketoesters. Chem. Commun. 2120 – 2121
Relevant patents 2000 - 2004
Duetz, W.A., and Witholt, B (2000). Method and apparatus for sampling frozen cultures of microorganisms and/or eukaryotic cells simultaneously and independently of each other, Patent Application PCT/NL00/00426
Duetz, W.A., Jourdat, C., Witholt, B. (2000). Process for the preparation of trans-carveol and/or carvone, PCT application PCT00124623.0, November 2000
Duetz, W.A., Jourdat, C., Witholt, B. (2001). Process for the preparation of perillyl alcohol, PCT application PCT01103785.0, February 2001
Relevant lectures 2000 - 2004
1st International Symposium on Synthesis, Screening and Sequencing, Achema, May 23-25, 2000, Frankfurt, Germany, Parallel growth, storage, and replication of large collections of aerobic microbial strains in microtiter plates: use for high throughput screening for new biocatalysts and secondary metabolites (Wouter Duetz)
2nd International Symposium on Applied Shaking technology, New York, April 2001. Oxygen transfer rates in square vessels and deepwell microtiter plates (Wouter Duetz)
Biochemical Engineering XII, Sonoma, San Fransisco, June 2001 Parallel growth, storage, and replication of large collections of aerobic microbial strains in microtiter plates: use for high throughput screening for new biocatalysts and secondary metabolites (Wouter Duetz)
NBC, Ede, The Netherlands, March 2002, Screening for hydroxylases in wild type strains: parallel growth, storage, and replication of large microbial collections in microtiter plates (Wouter Duetz)
ASM general meeting, Salt Lake City, May 2002, Use of a miniaturized microbial screening system for the discovery of new hydroxylation reactions (Wouter Duetz)
SGM meeting, Dublin, September 2003. Screening for microbial oxidoreductases; approaches and results
DECHEMA meeting, Eisenach, May 2004. "Simultane und integrierte Bioprozeßentwicklung": Parallel aerobic growth, storage, and replication of large microbial collections in microtiter plates