List of users, applications, and publications

(for which our technology platform was used):

 

note: this list is incomplete (still under construction)

 

Agroferm (Esbjerg, Denmark): Mutant screening and medium optimization (Corynebacterium)

 

Alpharma (Copenhagen, Denmark): mutant screenings and medium optimization (actinomycetes)

 

AMRI (Albany, NY, USA) high throughput screening for:

 

    * Rapid identification of microbial catalysts useful in select biotransformations,

    * Rapid identification of microbial catalysts useful in metabolite production,

    * Rapid growth medium, reaction condition and extraction optimization,

    * Streamlined process from mutagenesis to improved production strain.

 

Cubist pharmaceuticals (Lexington, USA): screening for new antibiotics produced by actinomycetes

 

· Baker, D.D. et al. (2007) The value of natural products to future pharmaceutical discovery. NATURAL PRODUCT REPORTS 24:1225-1244

 

Danisco / Genencor (Leiden, The Netherlands, Palo Alto, USA): Screening and fermentation of construct libraries in Schizosaccharomyces pombe at 96-lowwell mtp level.

 

ETH, Systems biology (Zurich, Switzerland): Quantitative flux analaysis in Escherichia coli, Bacillus subtilis and yeasts

 

· E. Fischer E, U. Sauer (2005). Large-scale in vivo flux analysis shows rigidity and suboptimal performance of Bacillus subtilis metabolism NATURE GENETICS 37: 636-640 .

· L.M. Blank, L. Kuepfer, U. Sauer (2005). Large-scale C-13-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast. GENOME BIOLOGY 6: Art. No. R49 .

· M. Sonderegger, M. Schumperli, U. Sauer (2005) Selection of quiescent Escherichia coli with high metabolic activity.METABOLIC ENGINEERING 7: 4-9 .

· E. Fischer, N. Zamboni, U.Sauer (2004) High-throughput metabolic flux analysis based on GC-MS derived 13C constraints (2004) Anal. Biochem. 325: 308-316.

· E. Fischer & U. Sauer (2003). A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry E. coli. J. Biol. Chem. 278: 46446-46451.

· P. Steiner & U. Sauer (2003) Overexpression of the ATP-dependent helicase RecG confers acetate tolerance to E. coli. Appl. Microbiol. Biotechnol. 63: 293-299.

· E. Fischer & U. Sauer (2003). Metabolic flux profiling of E. coli mutants in central carbon metabolism by GC-MS. Eur. J. Biochem. 270: 880-891.

· N. Zamboni & U. Sauer (2003). Knockout of the high-coupling cytochrome aa3 oxidase reduces TCA cycle fluxes in B. subtilis. FEMS Microbiol. Lett. 226:121-126.

 

Iogen (Ottawa, Canada): Screening of yeast libraries and growing fungal microcultures

 

Merck (Madrid, Spain): Screening of collections of fungi and actinomycetes for new secondary metabolites

 

· Bills, G.F. et al (2008) Enhancement of antibiotic and secondary metabolite detection from filamentous fungi by growth on nutritional arrays. Journal of Applied Microbiology, March 2008

 

Max-Planck Institute, Department of Chemical Biology (Dortmund, Germany): screening of new antibiotics based on different compound libraries

 

MIT (Boston, USA): cultivation of yeast libraries

 

Novartis Vaccines Research Centre (Sienna, Italy): early development

 

Novozymes (Copenhagen, Denmark): screening of fungal enzyme activities

 

Technical university Denmark (DTU, Biocentrum, Copenhagen): cultivation of yeasts and acinomycetes

 

Technical university Lisbon (Centre Biological and Chemical Engineering): bacterial cultivations

 

University Leiden,Gorlaeus Laboratories, Genexpress (the Netherlands): cultivation of libraries of streptomycetes

 

· Traag, B.A. (2007) Characterization of the sporulation control protein SsgA by use of an efficient method to create and screen random mutant libraries in streptomycetes. APPLIED AND ENVIRONMENTAL MICROBIOLOGY  73:2085-2092

 

University of Dortmund (Germany): Quantitative flux analaysis in Escherichia coli, Bacillus subtilis and yeasts

 

University of Dublin (Ireland): screening of mutant libraries of Pseudomonas strains

 

University of Genth (LIMAB): High-throughput screening of enzyme libraries expressed in E. coli

 

University of Graz,  Institute of Molecular Biotechnology (Austria): Replication of microbial libraries

 

University of Tübingen, Pharmaceutical Institute (Germany): Antibiotic production in Streptomyces